Search results for "Biuret test"

Evaluation and elimination of the “blank bias error” using the H-point standard addition method

1992

Abstract The basis of the H-point standard addition method in the cases where the analyte determination requires the use of an absorbent reagent is considered. The method evaluates and eliminates the blank bias error present in such procedures by using the absorbance increment at two selected wavelengths as the analytical signal of the calibration graphs. Three different determinations were tested: the determination of proteins with the biuret method, thorium with thoron and of magnesium with Titan Yellow. These procedures are better described by the proposed method than by the traditional approach using absorbance values against reagent blank.